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KMID : 0359319990390010118
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Korean Journal of Veterinary Research
1999 Volume.39 No. 1 p.118 ~ p.125
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Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction
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임숙경/Lim, Suk Kyung
이희수/우승룡/윤순식/문운경/이유영/고홍범/Lee, Hee Soo/Woo, Sung Ryong/Yoon, Soon Seek/Moon, Oun Kyong/Lee, Yoo Young/Koh, Hong Bum
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Abstract
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Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age.
PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs.
Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE.
Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.
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